HISTOCHEMICAL IDENTIFICATION OF ACID MUCOPOLYSACCHARIDES WITH ACRIDINE ORANGE
نویسندگان
چکیده
منابع مشابه
Histochemical observations on Mycoplasma after staining with acridine orange.
Mycoplasma colonies and Mycoplasma cells in preparations from infected milk and lymph nodes were observed for their fluorescent qualities after treatment with acridine orange. Mycoplasma colonies fluoresced brilliant red or red-orange. When treated after exposure to ribonuclease, the colonies fluoresced lime-green. There was no fluorescence when both ribonucleic acid and deoxyribonucleic acid w...
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The recent paper by Rotimi and colleagues 1 does not mention the acridine orange stain 2 when comparing staining methods for the identification of Helicobacter pylori. The acri-dine orange stain uses ultraviolet fluores-cence in the identification of bacteria. The typical curved morphology of H pylori can easily be diVerentiated from other bacteria. I have used this quick, cheap, and reliable s...
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A fluorescence quenching method was used to determine the equilibrium binding constants for the association of acridine, acridine orange, and acridine yellow G to humic acid. The fluorescence of each polycyclic aromatic nitrogen heterocycle (PANH) was monitored as aliquots of humic acid were added, and a Stern-Volmer plot was produced in which the slope is the equilibrium constant of the bindin...
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The in vitro localization of acridine orange (AO) in living cells was monitored by means of fluorescence microscopy, quantitative cell viability studies, and photofluorimetric measurements following dye-cell interaction. The parameters, pH, time, dye concentration, and the metabolic state of the cell were found to exert a profound influence on the time course and distribution of staining. The p...
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The triplet state of acridine orange dissolved in methanol/water matrix was investigated by ESR. In absence of oxygen a strong temperature dependence of the spectra was observed. At low temper ature (100 °K) the zero-field splitting parameters calculated from the triplet spectrum are: X /h c = 0.0050 cm-1 , Y / h c = 0.0342 cm-1 , Z //ic = 0.0387 cm-1 , at higher temperature (140 °K) : X * ( h...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1964
ISSN: 0022-1554,1551-5044
DOI: 10.1177/12.3.164